Transduction of the e1a2Mz expression vector into Ph⁺ ALL cells. (A) Schematic diagrams of the 4 constructs used to produce the e1a2Mz expression HIV(VSV) vector. pHIV-e1a2Mz, pMDLg/p.RRE, pRSV-rev, and pMD.G indicate the e1a2Mz-encoding transfer vector, packaging plasmid, rev expression plasmid, and VSV G-protein expression plasmid, respectively. P indicates tRNA^Val (pol III promoter of the e1a2Mz); MzL, e1a2Mz left arm; MzR, e1a2Mz right arm; CMV, cytomegalovirus promoter; LTR, long terminal repeat; ψ, packaging signal; SD, splice donor; SA, splice acceptor; RRE, Rev-responsive element; R, repeat region; ΔU3, promoter/enhancer sequence-deleted U3 (▨); cPPT, central polypurine tract; CTS, central termination sequence; EF-1α, elongation factor 1α subunit promoter; hCD2, human CD2; WPRE, woodchuck hepatitis virus posttranscriptional regulatory element; GAG, gag region of HIV-1; POL, pol region of HIV-1; pA, poly A; RSV, respiratory syncytial virus promoter; and VSV.G, vesicular stomatitis virus G protein. (B) The hCD2 expression in the Ph⁺ ALL cell line, KOPN-30. The hCD2 expression in KOPN-30 cells 3 days after transduction with control vector (ECD2; Table 1) or e1a2Mz expression vector (e1a2Mz; Table 1) at an MOI of 10 was determined by FCM. Histograms in solid lines indicate untransduced cells and the area under the solid lines represents vector-transduced cells. ^*The numbers above the bars indicate the percentage of hCD2-positive cells. (C) Transduction efficiencies into human leukemia cell lines, including 5 Ph⁺ ALL cell lines, with the ECD2 (▪) or e1a2Mz (▨) vector. The results represent means of triplicate experiments.