Effect of e1a2Mz transduction on the survival of Ph⁺ ALL cells. (A) Death of OM9;22 Ph⁺ ALL cells (p190-type bcr-abl positive) and K562 CML-BC cells (p190-type bcr-abl negative) transduced with the indicated vectors, or untransduced, was determined by the trypan blue exclusion test every 2 or 3 days. The results represent means of triplicate experiments. (B) Viabilities of leukemia cell lines transduced with the ECD2 (▪) or e1a2Mz (▨) vector on day 14. These results represent means ± SDs of triplicate experiments. (C) Apoptosis of the Ph⁺ ALL cell line KOPN-30 transduced with e1a2Mz. KOPN-30 cells transduced with the ECD2 or e1a2Mz vector were stained with FITC-labeled annexin V and PI on days 7 and 14 and were analyzed by 2-color FCM. Cells in each quadrant correspond to the following conditions: bottom left, live; bottom right, in early apoptosis; top right, in late apoptosis or dead. ^*The numbers in the plots indicate the percentage of cells in that quadrant. (D) Copy numbers of integrated lentiviral vector per cell transduced with the indicated vectors at an MOI of 0.1 (□), 1 (▪) or 10 (▨). As for the cells transduced at an MOI of 0.1, the results of CD2⁺ cells selected by magnetic bead separation are shown. These results represent mean ± SD of triplicate experiments.