Expression of the e1a2Mz and p190-type bcr-abl mRNA. (A) Expression of the left and right arms of e1a2Mz in KOPN-30 and KOPN-57 cells transduced with the indicated vectors was examined by RT-PCR using specific primer pairs. RT-PCR for GAPDH was performed as an internal control. The pHIV-e1a2Mz plasmid was used as a positive control for the PCR. The RT-PCR products were electrophoresed through a 2% agarose gel and were stained with ethidium bromide. (B) Quantification of p190-type bcr-abl mRNA in Ph⁺ ALL cell lines transduced with or without the e1a2Mz by real-time RT-PCR using specific primer pairs and probe. The copy number of bcr-abl mRNA was calculated from the standard curve. The results represent means ± SDs of triplicate experiments. ^*The amount of RNA was normalized by the copy number of GAPDH mRNA.