Effect of PTX3 overexpression on endothelial FGF2-T-MAE cell growth in vitro and in vivo. (A) Parental FGF2-T-MAE cells (•), PTX3-overexpressing clones B4 (▴), B8 (▵), B9 (▪), and B20 (○), and mock-transfected clones BVV6 (♦) and BVV10 (⋄) seeded at 10 000 cells/cm² in 24-well plates were grown and counted at the indicated periods of time. Each point is the mean of 2 determinations in duplicate. (Inset) Linear regression of the number of cells measured at day 8 versus the levels of PTX3 production (ng PTX3/5 × 10⁶ cells/48 hours) (r = 0.88; P < .01). (B) Parental (•), EGFP-infected (▪), and PTX3-infected FGF2-T-MAE cells (○) were injected subcutaneously in nude mice, and the size of the growing tumors was measured. Each point is the mean ± SD of 8 animals. *Statistically different from parental plus EGFP-infected FGF2-T-MAE lesions (Student t test, P < .05).