Figure 5.
Effect of IGFBP-3. Retinoid (A-B) or 1,25(OH)2 vitamin D3 (C) induced the expression of CD11b on HL-60 (A, C) and NB4 (B) cells. HL-60 (A) and NB4 (B) cells were cultured for 2 days with IGFBP-3 (5 μg/mL), a retinoid (10–8 or 10–7 M), or both in combination and then were analyzed by FACscan for CD11b expression. (C) HL-60 cells were cultured for 2 days with IGFBP-3 (5 μg/mL), 1,25(OH)2 vitamin D3 (10–8 M), or both in combination and then were analyzed by FACscan for CD11b expression. Data represent the mean ± SD of triplicate cultures, and results were derived from 2 independent experiments. RAR496 indicates AGN197496; RXR204, AGN194204; BP-3, IGFBP-3.
