Figure 6.
![Figure 6. Analysis of AA metabolites from AA-treated platelets by thin-layer chromatography (TLC). Washed platelets from 2 to 3 mice were used for each experiment. Five microliters of [1-14C]AA (0.1 mCi/mL [3.7 MBq/mL]) was added to washed platelets and incubated at 37° C for 10 minutes. The reaction was stopped by 27 μL 1 N HCl. Prostanoids were extracted with diethyl-ether before dissolving in 50 μL chloroform for TLC analysis. Developing condition was at room temperature for 80 minutes in 30 mL chloroform-methanol–acetic acid–water ratio of 90:8:1:0.8 (vol/vol/vol/vol). Commercially obtained metabolites were included as references (marked at the left hand side). +/+ indicates WT mice; +/–, heterozygous; and –/–, homozygous mutant.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/104/1/10.1182_blood-2003-10-3661/6/zh80130463390006.jpeg?Expires=1735850637&Signature=vFRolqr8o610B3uFl47R~umQLWekrN9bSy-HNNVDU4oc~uqsHOELN11xFcgzFuOd-j5RyZGMNM8OKPmC5Lw9PmS5EONnZeXNecQCdvhIUlTgSIRsjQ6lVJ3NZ73r8zYNnkj7yHor3vZXTmSz4~oqQ6Nuy~sQLZBvpcUf-df3ByNRiSihvF9WN52m1mNTHHaJDZ9mwA~5feoYAJfulbqTPjyTrx8~32-VEk6YnwlKbMfomoW8d9Gogs5YVA950XTpEuEeeeK1EbtZuX9jQOmLvq6307SeCDxrVnB4sFNHalf~NDlAgmO9GxQW2Lz8ENjqQMaFwVebbEDJMcCcuFfdlA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Analysis of AA metabolites from AA-treated platelets by thin-layer chromatography (TLC). Washed platelets from 2 to 3 mice were used for each experiment. Five microliters of [1-14C]AA (0.1 mCi/mL [3.7 MBq/mL]) was added to washed platelets and incubated at 37° C for 10 minutes. The reaction was stopped by 27 μL 1 N HCl. Prostanoids were extracted with diethyl-ether before dissolving in 50 μL chloroform for TLC analysis. Developing condition was at room temperature for 80 minutes in 30 mL chloroform-methanol–acetic acid–water ratio of 90:8:1:0.8 (vol/vol/vol/vol). Commercially obtained metabolites were included as references (marked at the left hand side). +/+ indicates WT mice; +/–, heterozygous; and –/–, homozygous mutant.
