Figure 2.
IL-8 and TNF-α induced the release of ULVWF from HUVECs. (A) HUVECs were treated with various concentrations of cytokines for 30 minutes and then perfused with washed platelets suspended in Tyrode buffer at 2.5 dyne/cm2. The ULVWF released from HUVECs stimulated by IL-8 or TNF-α had maximal effects at 100 and 10 ng/mL, respectively (mean ± SEM, Student t test, n = 9). (B) HUVECs were treated with 50 ng/mL TNF-α for up to 24 hours before perfusion with washed platelets. The formation of ULVWF strings increased with incubation times, reaching a plateau after 60 minutes (mean ± SEM, n = 4).
