Figure 3.
Representative photomicrographs of rat mesenteric arterioles and venules in animals untreated and treated with Ang II. Arteriolar endothelium after 4-hour saline (A) or Ang II (B) exposure. CD3+ staining of leukocytes adhered to the arteriolar endothelium after 4-hour Ang II exposure (C). ED1-positive staining of leukocytes adhered to the arteriolar endothelium after 4-hour Ang II exposure (D). ED1-positive staining of a leukocyte emigrating through the arteriolar endothelium after 4-hour Ang II exposure (E). Video photomicrographs of the rat mesenteric arterioles after 24-hour buffer intraperitoneal injection (F). Video photomicrographs of the rat mesenteric arterioles after 24-hour Ang II intraperitoneal injection (G). Venular endothelium after 4-hour saline (H) or Ang II exposure (I). A 100×/1.25 oil objective lens was used for panels A-E and H-I; a 20×/0.40 objective lens was used for panels F-G. Panels A, B, H, and I were stained with hematoxylin/eosin, and panels C, D, and E were lightly counterstained with hematoxylin. Leica IM1000 software capture imaging (Leica Microsystems, Wetzlar, Germany) was used to obtain the images in panels A-E and H-I, and a Sony color video printer was used for the images in panels F and G.