Intrahepatic CD4⁺CD8⁺ cells in HCV infection. (A) Liver histology (original magnification × 200) showing chronic hepatitis C, double stained for both CD4 (brown chromogen) and CD8 (red chromogen). T cells are clustered around the lymphoid aggregate and at the edges of the portal area where they are involved in interface hepatitis. The arrows indicate 2 CD4/CD8 double-stained cells. (B-D) High-magnification (original magnification × 1000) photomicrographs of double-stained cells in portal areas and hepatic sinuses. The arrows indicate CD4/CD8 double-stained cells.