Induction of MCP-1 secretion by fibrinogen-bound IL-1β Endothelial cells were plated on gelatin-coated wells in McCoy 5A medium supplemented with 20% FBS, 50 μg/mL ECGS, and 100 μg/mL heparin and allowed to reach confluence. The cells were washed twice with McCoy medium and incubated with 2 ng/mL of IL-1β in the presence or absence of 1 μg/mL fibrinogen for 2 hours. The medium was then collected, and MCP-1 was measured by ELISA. The results shown are the mean ± SD of 3 separate experiments.