VEGF can induce increased phosphorylation of VEGF-R1 and VEGF-R2 receptors on CLL B cells, and phosphorylation of these receptors can be inhibited by EGCG. Primary CLL B cells were cultured in the presence or absence of EGCG for 24 hours and then stimulated with VEGF₁₆₅ (50 ng/mL) for an additional 30 minutes prior to preparation of the protein extract. Protein extract from the immunoprecipitation (500 μg) was subjected to SDS-PAGE and transferred to nitrocellulose. The blots were initially probed with the p-Tyr antibody and then stripped with erase buffer. The blots were then reprobed with the various antibodies of interest. The data are representative of 1 of 3 different experiments. IP indicates immunoprecipitation.