Effects of EGCG on Mcl-1 and XIAP proteins in CLL B cells. (A) Representative results for sensitive CLL B cells when EGCG is added to the majority of CLL B-cell cultures (n = 8 of 10). Thus, PARP cleavage decreases in Mcl-1, XIAP, and caspase-3, and little or no change in Bcl-2 is seen. Freshly isolated CLL B cells (n = 8 patients) were treated with either vehicle or EGCG for 24 hours. Protein extracts (50 μg) were prepared and subjected to SDS-PAGE, following transfer to nitrocellulose. The blot was probed with the antibodies of interest. (B) Representative blot for the EGCG-insensitive CLL B-cell populations. Note there was no change in the apoptotic proteins or PARP cleavage in the insensitive CLL patients.