Increased chemotactic response to CXCL12 of PMNs and T lymphocytes in WHIM patients. (A) T-cell chemotaxis in response to CXCL12 (10, 100, 500, 1000, 1500 ng/mL), CCL21 (50 ng/mL), or CCL2 (50 ng/mL) was assessed in WHIM patient P3 (• and ▪) or healthy subject (○ and □) as described in “Patients, materials, and methods.” (B) PMN chemotaxis in response to CXCL12 (10, 100, 1000 ng/mL) or to CXCL8 (50 ng/mL) was assessed in WHIM patient P3 (• and ▪) or healthy subject (○ and □) as described in “Patients, materials, and methods.” (C-D) T-cell transendothelial migration (C) and T-cell adhesion (D) in response to CXCL12 (10, 100, 500 ng/mL) were assessed in WHIM patient P1 (▪) and in a healthy subject (□) as described in “Patients, materials, and methods.” CXCL12-induced transmigration and adhesion at the net of spontaneous activity are presented as percent of input cells. In panels A and B, results are presented as chemotaxis index and are representative of 2 other experiments performed in patients P2 and P3 with similar results. Asterisks (^*) indicate a significant difference in chemotactic response of cells from a WHIM patient in comparison to cells from a control subject (P < .05). Error bars indicate SD.