Peripheral blood T-lymphocyte phenotype of WHIM patients. (A) Naive, central memory (CEM), effector memory (TEM), and effector memory RA⁺ (TEMRA) T lymphocytes, whole-blood cells from WHIM patients (P1, P2, P3), and 9 age-matched controls identified by triple staining for the expression of CD4 or CD8 and both CCR7 and CD45R0. (B) FACS analysis was performed by gating on CD4⁺ or CD8⁺ cells. (C) Bars represent the relative proportions of the 4 T-cell subsets obtained by flow cytometry. (D) Squares indicate the average absolute number of lymphocytes per microliter for each subset in WHIM patients (▪) and controls (□).