Separation of CD34⁺/CD38^– cells into SDF and FDF. CD34⁺/CD38^– and CD34⁺/CD38⁺ subpopulations were isolated from human CB (A). Reanalysis of the sorted cells confirmed the efficient separation of these cell fractions (B-C). The CD34⁺/CD38^– stem cell population was then stained with PKH26 and cultivated for 1 week. The SDF (PKH26⁺) was separated by cell sorting form the FDF (PKH26^–) population (D). Efficient enrichment was controlled by reanalysis (E-F).