Confirmation of differential expression by real-time RT-PCR. Semiquantitative RT-PCR by LightCycler analysis was used to verify differential gene expression of 7 genes in FDF and SDF in relation to 18s rRNA. HLA-A and CD133 were higher expressed in the SDF. γ-Globin, CD36, and cadherin-e (not shown) were more highly expressed in the FDF. GAPDH and ubiquitin did not show significant differential expression and were used as additional controls. All figures belong to the same run of 1 RNA sample. A high correlation between RT-PCR and microarray analysis was observed. Error bars represent SD of at least 3 experiments.