Figure 4.
SDF has fibroblastoid morphology. The SDF (A-B) and the FDF (C-D) were separated according to their PKH26 staining after 1 week. Cells in the SDF are on average smaller; 69% of them revealed membrane protrusions. In contrast, cells in the FDF had a round morphology and only 16% displayed podia formation. CD133 (prominin) is more strongly expressed in the SDF and accumulates at the tip of lamellipodia (E-F; G-H; red = PKH26; green = CD133). Error bars represent SD. Cells were observed with a 40× immersion objective (40× HI; Olympus Optical, Hamburg, Germany), microscope model IX-70 (Olympus Optical) connected to a video camera (Colorview XS; Soft Images Systems, Münster, Germany; resolution: pixel size 6.7 × 6.7 μm; NA of 40 × objective: 1.0).