Effects of distinct cannabinoids on the G-CSF-induced neutrophilic differentiation and migration of 32D/G-CSF-R cells. (A) Morphologic analysis of May-Grünwald Giemsa-stained cytospins of 1 representative Cb2-expressing clone cultured in G-CSF and 100 nM 2-AG or CP55940 (CP) in the presence or absence of 1 μM Cb2 (C2) or Cb1 (C1) inverse agonist. In vitro migration of 32D/G-CSF-R/Cb2 cells upon 2-AG or CP stimulation; 100 nM C2 or C1 was added to the upper well when tested. (B) Two representative Cb2-expressing and 2 representative EGFP-expressing clones were cultured in G-CSF and 100 nM 2-AG or CP. (C) Differential counts of a representative CP titration experiment in the presence of G-CSF (day 8 of culture). □ indicates blast cells; ▪, intermediate forms; and ▦, terminally differentiated neutrophils. (D) Differential counts of a 32D/G-CSF-R/Cb2 clone cultured with G-CSF, 100 nM CP, and different concentrations of C2.