Effect of pertussis toxin (PTX) on the CP55940-evoked block of differentiation and the 2-AG-induced migration of 32D/G-CSF-R cells. (A) Two representative Cb2- and EGFP-expressing 32D/G-CSF-R clones were cultured for 8 days in the presence of G-CSF plus CP559940 (CP) with or without PTX (100 ng/mL). (B) Differential counts of a representative Cb2- and EGFP-expressing 32D/G-CSF-R clone at day 7 of culture in the presence of G-CSF with or without CP and PTX. □ indicates blast cells; ▪, intermediate forms; and ▦, terminally differentiated neutrophils. (C) Effect of 300 ng/mL PTX on 2-AG-induced migration of 32D/G-CSF-R/Cb2 cells. PTX was added to the cells and preincubated for 1 hour at 37°C before the cells were placed in the upper chamber of a transwell assay. Values indicate the average of 3 representative clones. Error bars indicate SD.