Effects of dbcAMP and MEK/ERK inhibitors on 2-AG-induced migration and the CP55940-stimulated block of differentiation. (A) A representative Cb2 and EGFP control 32D/G-CSF-R clone cultured with G-CSF with or without 100 nM CP55940 (CP), 100 μM dbcAMP, or 25 μM PD98059 (PD). (B) Pictures of May-Grünwald Giemsa-stained cytospins (day 8) of a representative Cb2-expressing 32D/G-CSF-R clone cultured under the different conditions. Original magnification × 63. (C) Effects of different concentrations of dbcAMP and U0126 on the 2-AG-induced (300 nM) migration of a representative Cb2-expressing 32D/G-CSF-R clone. The y-axis indicates the percentage of migrated cells in relation to the nontreated cells.