HCMV gene expression in hematopoietic subpopulations. CD34⁺ subpopulations infected with TLsubUL21.5 at an MOI of 5 PFU/cell were purified at 20 hours after infection by cell sorting and seeded in long-term bone marrow cultures. Linearly amplified RNAs were analyzed by using the HCMV array over a time course. All RNAs were hybridized in triplicate. Representative arrays hybridized with RNA derived from infected subpopulations at 5 days after infection are shown. Arabidopsis cDNA spots used as positive controls are circled. Cellular cDNA spots are boxed. All other spots represent HCMV genes.