Figure 4.
Figure 4. Cells expressing a stem cell phenotype support a productive infection in long-term culture. TLsubUL21.5-infected (A) CD34+/CD38–/lin–/Thy-1+ or (B) CD34+/CD38– cells were purified by cell sorting at 20 hours after infection and seeded into long-term bone marrow cultures. Linearly amplified RNAs were analyzed by using the HCMV array over a time course. The arrays shown are representative of 2 independent experiments in which triplicate arrays were hybridized with RNAs derived from cells at (A) 5 and 20 or (B) 10 and 20 days after infection for CD34+/CD38–/lin–/Thy-1+ and CD34+/CD38– cells, respectively. Arabidopsis cDNA spots used as positive controls are circled. Cellular cDNA spots are boxed. All other spots represent HCMV genes.

Cells expressing a stem cell phenotype support a productive infection in long-term culture. TLsubUL21.5-infected (A) CD34+/CD38/lin/Thy-1+ or (B) CD34+/CD38 cells were purified by cell sorting at 20 hours after infection and seeded into long-term bone marrow cultures. Linearly amplified RNAs were analyzed by using the HCMV array over a time course. The arrays shown are representative of 2 independent experiments in which triplicate arrays were hybridized with RNAs derived from cells at (A) 5 and 20 or (B) 10 and 20 days after infection for CD34+/CD38/lin/Thy-1+ and CD34+/CD38 cells, respectively. Arabidopsis cDNA spots used as positive controls are circled. Cellular cDNA spots are boxed. All other spots represent HCMV genes.

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