Genotyping of Mart alloantigens by allele-specific PCR (PCR-SSP).PCR was performed with a common intronic antisense primer and an allele-specific sense primer (G230 or A230) (top). Representative results of Mart genotypes determined by PCR-SSP of 3 donors: homozygous Mart-positive (G/G) (lane 2), heterozygous Mart-positive (G/A) (lane 3), and homozygous Mart-negative (A/A) (lane 4). Genomic DNA was amplified using primer for A230 or G230 and was analyzed by 1.8% agarose gel electrophoresis. The 249-bp bands represent the allele-specific product, and the hGH bands represent the internal control. DNA fragments VI (Roche) were used as standards (lane 1).