Figure 4.
Influence of anti-Mart in platelet-neutrophil aggregate formation in whole blood from donors who underwent Mart genotyping. The effect of anti-Mart IgG (50 μL/mL) derived from 2 different sera (sera 1-2) and Fab fragments of a blocking mAb GA6* (10 μg/mL) against P-selectin on platelet-neutrophil adhesion in whole blood stimulated with the stable thromboxane A2 mimetic U46619 (5 μM) was tested. Adhesion experiments were carried out in a rheometer at shear rates of 20 s–1 (low shear) and 2000 s–1 (high shear). Data represent the mean ± SD of 3 independent experiments.