MAPK family members do not mediate CCR7-dependent inhibition of apoptosis. DCs were left untreated (control) or pretreated with UO126 (5 μM), SB203580 (13 μM), or SP600125 (50 μM) for 60 minutes to inhibit, respectively, Erk1/2, p38, and JNK. Parallel control experiments demonstrated that these enzymes were completely blocked by the respective inhibitors (not shown). Subsequently, DCs were left unstimulated (–) or stimulated (+) with CCL19 or CCL21. (Bottom) After 2.5 minutes of stimulation with chemokines, aliquots were taken to analyze the level of active Akt1 (p-Akt) and total Akt1 by Western blotting. A representative experiment out of 3 performed is shown. (Top) Remaining DCs were left for an additional 6 hours, and then the percentage of annexin-positive/7-AAD–negative cells was quantified. The results represent the mean ± SEM of 3 independent experiments.