The role of NK cells in engraftment of purified HSCs. (A) Shading represents MHC disparity between donor and recipients. (B) Kaplan-Meier curves show the survival of B6, B6/Beige, or NK cell–depleted B6 recipients of B10.BR HSCs with or without B10.BR FCs. (C) Survival curve of B10.D2 HSCs into B10.BR or NK cell–depleted B10.BR recipients. (D) Multilineage typing of representative B6/Beige recipients of B10.BR HSCs alone compared with (E) normal B6 recipients of B10.BR HSCs plus B10.BR FCs. Multilineage data are from peripheral blood 3 months after transplantation and analyzed based on the lymphoid gate. Data shown are from one representative recipient. A total of 3 to 4 recipients were analyzed per group.