Isolated platelets in plasma-deficient reconstituted blood express VN on their surface after perfusion over a fibrin network. Perfusion studies were performed for 5 minutes at a shear rate of 500 s^–1 with reconstituted blood containing human albumin solution instead of plasma on fibrin plus multimeric VN (A, C, E) or fibrin (B, D, F) fibers. After perfusion, coverslips were processed for confocal laser scanning microscopy (original magnification × 250): VN was visualized with an FITC-labeled Poab (green, A-B), and platelets were labeled with TRITC-phallacidin (red, C-D). The merged signal is shown in yellow (E-F). White bar represents 25 μm.