Figure 5.
Anti-fVIII ELISA titers in hemophilia A mice immunized with HSQ and HSQ R484A/R489A/P492A. Plasma from the HSQ and HSQ R484A/R489A/P492A groups described in Figure 4 were tested for anti-fVIII antibodies by ELISA using plates coated with isologous antigen, as described in “Materials and methods.” Horizontal lines represent the sample means.