Fucoidan induces the release of proteases without activation of myeloid cells. (A) BMNCs from αM^+/+, αM^–/–, or C3H/HeJ mice were treated with 100 μg/mL fucoidan, vehicle, or with 50 ng/mL PMA (C3H/HeJ group only). Enzymatic activities were measured using a chromogenic substrate. Error bars represent mean ± SEM relative elastase activity. *P < .01; **P < .05, n = 4. (B) Expression of L-selectin or αM in vehicle, fucoidan, and PMA-treated BMNCs and (C) the oxidative burst as measured with DCFDA were analyzed by flow cytometry. Vertical lines indicate the fluorescence level of untreated or negative control samples. Shown are representative of at least 3 experiments.