Lytic activity, IFN-γ release, and tetramer binding in pp65/pp150-MVA IVS cultures. IVS using pp65/pp150-MVA was performed with HLA A^*0201 donors 001, 009, 011, and 007, and for HLA B^*0702. (A) Cytotoxic activity detected after IVS is shown for each donor. ▦ indicates background lysis to autologous LCLs loaded with p53_149-157; ♦ indicates lysis of autologous LCLs pulsed with pp65_495-503 (donors 001, 009, and 011) or pp65_417-426 (donor 007); • indicates lysis of pp150_945-955-pulsed autologous LCLs of donor 011. (B) Tetramer binding levels in CD8⁺ cells from fresh PBMCs (○) and pp65/pp150-MVA IVS cultures (•). pp65_495-503 tetramers were used for donors 001, 009, and 011, while donor 007 was tested using pp65_417-426 tetramers. CD8⁺ T-cell binding to HIV pol_464-472 tetramers was subtracted. (C) Percentage IFN-γ release from CD8⁺ cells of fresh PBMCs (○) and pp65/pp150-MVA IVS cultures (•) detected in ICC. Peptides used during ICC incubation were p53_149-157 and pp65_495-503 for HLA A^*0201 donors and pp65_417-426 for HLA A^*0702 donor 007. For each donor, percentages of IFN-γ CD8⁺ cells to irrelevant p53_149-157 peptide were subtracted from the corresponding specific values.