Expression of NUP98-TOP1 in murine bone marrow confers in vitro proliferative advantage. (A) NUP98-TOP1 expression results in expansion of transduced (GFP⁺) BM cells in liquid cultures. Graph is representative of 3 independent experiments. (B) Expression of NUP98-TOP1 leads to decreased doubling time (Td) calculated from the slope of the curve representing change in cell number over time. (C) Representative Wright-Giemsa-stained cytospin preparation of GFP cytotoxic T lymphocyte (CTL; left) or NUP98-TOP1 (right) transduced bone marrow cultured for 4 weeks (original magnification, × 600).