Figure 2.
Effect of in vivo IFN-γ treatment on primitive and mature progenitors in the bone marrow. Cells from WT and Fancc-/- mice that were treated with IFN-γ or vehicle control were cultured at 2 × 104 LDMNCs/mL for the growth of high proliferative potential colony forming cells (HPP-CFCs) and low proliferative potential colony forming cells (LPP-CFCs). Each condition was plated in triplicate and scored on day 7 for LPP-CFCs and day 14 for HPP-CFCs. The dosage of IFN-γ that was administrated and the mouse genotypes are indicated. Error bars represent the standard error of the mean (SEM). *P < .001 comparing the genotypic differences in the reduction of clonogenic formation (n = 5).
