American Society of Hematology

Figure 1.

$Figure 1. Enriched human PMNs do not express granzymes A, B, or perforin. (A) Scatter and density plots for lineage-specific surface markers (CD3, CD15) and intracellular granzymes (GzmA and GzmB). Purified fractions were stained as indicated and a total of 10 000 events were acquired in the indicated gates. Data are representative of 1 of 2 donors. The numbers in the upper right corner of each panel indicate the percentage of events in the respective quadrants. (B) Purified PBMC (lanes 2-5) and PMN (lanes 6-9) lysates from 4 donors were blotted as indicated. Isolated GzmA, GzmB, or PFN (100 ng) served as positive control (lane 1). (C) PBMC and PMN lysates from 4 donors were assayed for GzmA and GzmB by EIA. Values represent concentration of granzyme (ng/mL) in lysate.$

Enriched human PMNs do not express granzymes A, B, or perforin. (A) Scatter and density plots for lineage-specific surface markers (CD3, CD15) and intracellular granzymes (GzmA and GzmB). Purified fractions were stained as indicated and a total of 10 000 events were acquired in the indicated gates. Data are representative of 1 of 2 donors. The numbers in the upper right corner of each panel indicate the percentage of events in the respective quadrants. (B) Purified PBMC (lanes 2-5) and PMN (lanes 6-9) lysates from 4 donors were blotted as indicated. Isolated GzmA, GzmB, or PFN (100 ng) served as positive control (lane 1). (C) PBMC and PMN lysates from 4 donors were assayed for GzmA and GzmB by EIA. Values represent concentration of granzyme (ng/mL) in lysate.

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