IL-2 cytokine release from cultured splenocytes and lymph node cells isolated from DO11.10 mice 10, 30, or 60 days after gene delivery. Individual spleens (A-C, n = 4-8) or pooled inguinal nodes (D-F) from GFP- or ova-transduced mice were cultured in the presence of 100 μg ova/mL media for 3 days. Error bars represent SD. *P less than .05 for ova-versus GFP-transduced mice. • indicates cells from ova-transduced mice stimulated with ova antigen; ▪, cells from GFP-transduced mice stimulated with ova; ○ and □, mock-stimulated controls. (G) Proliferation of cultured splenocytes as determined by measurement of 3H-tymidine incorporation (day 60 after vector administration, n = 4/group). Splenocyte proliferation was measured in the presence or absence (mock) of ova in conditioned media. For cells from AAV-ova–transduced mice, cells were also cultured in the presence of murine IL-2 (50 U/mL) in a parallel experiment. Error bars represent SD. *P ≤ .05.
