Figure 2.
IMC-EB10 binds to cell-surface FLT3 receptor in leukemia cells. Cells were incubated on ice with IMC-EB10 (solid line) or human IgG isotype control (dotted line) and then with PE-conjugated antihuman F(ab′)2 antibody. IMC-EB10 bound to the EOL-1 and BaF3-ITD cell lines, but not to the FLT3-negative cell lines JM1 or BaF3-control. Preincubation of EOL-1 or BaF3-ITD cells for 30 minutes on ice with FL (100 ng/mL) before antibody staining abrogated IMC-EB10 binding to cell surface FLT3 receptor.
