IMC-EB10 inhibits proliferation of EOL-1 and BaF3-ITD cells. (A) EOL-1 cells were starved in serum-free medium overnight. Cells were resuspended in AIM-V medium and were incubated for 68 hours with varying concentrations of antibodies (0-100 nM) in the presence or absence of exogenous FL (30 ng/mL). In a background control, cells were incubated with medium alone in the absence of exogenous FL. Cell proliferation was measured by [³H]-thymidine incorporation. For EOL-1 cells, background cycle per minute was deduced from the cycles per minute of all experimental samples. Percentage inhibition of FL-induced cellular proliferation was calculated as follows: [(cpm of untreated sample - cpm of antibody-treated sample)/cpm of untreated sample] × 100%. (B) Proliferation assay with BaF3-ITD cells was performed in RPMI 1640 supplemented with 10% FCS in the absence of exogenous FL for all samples. Error bars represent means ± SD.