Figure 3.
Effect of patient C anti-EPCR autoantibody on kinetic parameters of APC generation by endothelial cells. Thrombin-catalyzed protein C activation on EA.hy926 cells measured in the absence (•) or presence (○) of patient C IgM fraction at 45 μg/mL. The Km for each condition is indicated. The Lineweaver-Burk plot in the inset shows that the Vmax in both conditions is essentially equal.