Diploid controls for Aurora-B kinase localization in mitosis. (A) Diploid CD34⁺ cells showing expected pattern of Aurora-B kinase localizing to centromeres in prophase through metaphase, then transferring to the midzone cortex and the condensing midbody in anaphase. Note chromosomal organization defining the different stages with condensation beginning at prophase, chromosomal alignment occurring at metaphase, sister chromatid separation in early anaphase with spindle elongation, and the beginning of chromosome decondensation in late anaphase and telophase. (B) Comparison of a diploid metaphase (bipolar spindle, single metaphase plate) with a polyploid metaphase (more than 2 spindle poles and more than a single metaphase plate).