Western blot for caspase-12 in megakaryocytes. Protein lysates (20 μg) from large polyploid NF-E2^+/+ (lane 1) and NF-E2^–/– (lane 2) megakaryocytes were separated by SDS-PAGE (10%), and caspase-12 was detected by Western blot after transfer of proteins to nitrocellulose membrane. Lane 3 contains protein lysate (20 μg) from CD41⁺, VWF⁺ early megakaryocyte-lineage cells from wild-type mice.²⁷  The NF-E2 genotype is indicated below each lane, molecular weight markers (kDa) are indicated on the left, and caspase-12 (arrow) and caspase-12 cleavage products (bar) are indicated on the right.