Soluble fibrinogen binding following treatment with protease inhibitors. Soluble FITC-fibrinogen binding was determined following agonist stimulation of platelets pretreated with the caspase inhibitor ZVAD. Control platelets for each inhibitor, treated with carrier buffer only, are indicated by a horizontal bar. The amount of bound fibrinogen was determined by the mean fluorescence in the FL1 channel. Data presented are means ± SEM for 3 independent experiments. There is no significant difference between untreated and treated platelets at either ZVAD concentration tested.