Enhanced vascular leakage in the inflamed skin of VEGF-A transgenic mice. Induction of DTH reactions in the right ears of wild-type mice resulted in moderately increased vascular leakage, as visualized by the extravasation of Evans blue dye (A) which normalized within 7 days (C). Highly increased vascular permeability in the right ear of a VEGF-A transgenic mouse 24 hours after antigen challenge (B) and persistent though reduced Evans blue dye extravasation 7 days thereafter (D). Quantitative analysis of Evans blue dye deposition revealed enhanced vascular leakage in VEGF-A transgenic mice 24 hours (E) and 7 days (F) after elicitation of DTH reactions. Data are expressed as mean plus or minus SEM. *P < .05, **P < .01.