Reconstitution of B-cell numbers in MBS-treated mice. Total numbers for B-cell subsets, based on the developmental schema of Li and colleagues,48 were calculated using flow cytometry percentages and total cell counts. (Ai-ii) Combined results for BM and spleen, respectively, from experiments 1 to 3. (Aiii) Peritoneal B-lineage analysis was performed for 1 experiment, and the results from 1 animal from each control category and 2 MBS-treated animals are shown. MBS-treated mice exhibited recovery of all peritoneal B-cell subsets. *Statistically significant differences between MBS-treated mice and mock-transduced BtkTec–/– controls (with P values as noted). (B) Analysis of BM and spleen B-cell subsets from animals reconstituted with MBS-versus MIG-transduced stem cells. The numbers for each B-cell subset are shown, and mean total B-cell numbers with SEM are included at the top of each bar. *Statistically significant differences between MBS-treated mice and MIG-treated animals (with P values as noted).
