Comparison of the transcriptions of 141 angiogenesis-associated genes between HUAECs with and without treatment of ephrinB2 by cDNA microarray. The cDNA microarray membrane contains 141 gene clones. A total of 2.5 μg mRNA from HUAECs treated with 200 ng/mL preclustered ephrinB2 or 2 μg/mL clustering Ab alone for 4 hours was labeled with biotin-16–deoxyuridine triphosphate (biotin-16-dUTP) by reverse transcription and hybridized to the cDNA membrane, followed by color development. The genes that appeared to be upregulated are listed in Table 1. The arrowhead indicates β-actin (array no. C08), and the arrow indicates syntenin (array no. L08).