EphrinB2 inhibits bFGF-induced proliferation and migration of HUAECs. (A) Subconfluent HUAECs at a density of 5 × 10³ cells per well per 500 μL were plated into 24-well culture plates in the M199 medium containing 5% fetal calf serum (FCS) and 1 of 4 different regimens: clustering Ab alone (Control), 10 ng/mL bFGF, 200 ng/mL preclustered ephrinB2, or a combination of ephrinB2 and bFGF. The cultures were incubated for 3 days and then assessed by MTT assays. The samples of each tested group were in sextuplicate, and the experiment was performed in triplicate. Each data set was normalized with the average value of the negative control group. Bars represent the mean ± SD from 6 data of 1 representative experiment. (B) Quantification of migrated HUAECs. HUAECs were incubated with the indicated reagents for 6 hours in a modified Boyden chamber. After incubation, migrated cells were fixed and then stained with Giemsa solution. The migrated cells were counted at × 100 magnification. Three fields were counted in each well, and the average number was obtained. Each group was tested in sextuplicate, and tests were performed in triplicate. Each data set was normalized with the average value of the negative control group. Bars represent the mean ± SD from 6 results of 1 representative experiment. *P < .05 significantly greater than bFGF plus ephrinB2 group. #P < .05 significantly smaller than bFGF plus ephrinB2 group.