Strength and persistence of stimulation with intactE colimodulateMoDC migration and cytokine secretion. MoDCs were stimulated with intact E.coli as indicated. One half of the cultures were washed twice 3 hours after addition of Ecoli and then cultured in RPMI/FCS until 48 hours (mean ± SEM of 8 experiments are shown [n = 4 for E coli 1:1000]). (A) Migration toward CCL21 and (C) IL-12p70 secretion or (E) IL-6 secretion of MoDCs activated with varying concentrations of intact E coli. (*P < .05; **P < .01 as compared with E coli 1:1). Effect of washing MoDCs following 3 hours of activation with varying concentrations of live E coli on (B) migration toward CCL21 and (D) IL-12p70 secretion or (F) IL-6 secretion. Cultures were continued in RPMI/FCS for a total of 48 hours. Mean ± SEM of 8 experiments are shown (n = 4 for E coli 1:1000), *P < .05; **P < .01 compared with unwashed MoDCs (normalized to 1).