Kinetics of NTBI uptake from ferric citrate by hepatocytes. Hepatocytes from Hfe KO (filled symbols) and control (open symbols) mice were incubated for 0 to 90 minutes with ⁵⁹Fe-citrate (ratio of 10 μM Fe to 1000 μM citrate) at 37°C. (A) Internalized (•, ○), (B) membrane (▪, □), and (C) total (▴, ▵) uptake are shown. Results are expressed as mean ± SD (n = 3). This graph is a typical representation from 7 independent experiments performed.