The effects of divalent metals on internalization and membrane ferric citrate uptake. (A) Internalized. (B) Membrane. Hepatocytes from Hfe KO mice (▪) and control (□) were incubated with ⁵⁹Fe-citrate (ratio of 1 μM Fe to 100 μM citrate) in the absence and presence of 100 μM divalent metals for 1 hour at 37° C. Results are expressed as mean ± SD (n = 3). *P < .001; #P < .05. This graph is a typical representation from 3 independent experiments performed.