Expression of DMT1 mRNA and protein. (A) mRNA. (B) Protein. Messenger RNA and protein expression of DMT1 in the hepatocytes from Hfe KO (▪) and control (□) mice were quantified by real-time PCR and Western blotting, respectively, and performed as described in the “Materials and methods.” Results are expressed as mean ± SD (n = 6-8) for real-time PCR and mean ± SD (n = 7) for Western blotting. *P < .01.