Immunohistology of mouse aortas using antifusion protein mAb (antihirudin for Hir-Tg and antihuman TFPI for TFPI-Tg). All sections stained with DAPI (4,6 diamidino-2-phenylindole) nuclear stain (blue). (A) Expression of the fusion proteins is restricted to the intima. Antifusion protein mAb (green) contrasting with anti–α-actin mAb (red) used to stain media (original magnification × 100). (B) Colocalization of fusion proteins (green) with P-selectin (red) in ECs of the transgenic mice (original magnification × 100).