PKC inhibitors selectively block thrombin-stimulated permeability increases in ECs. Cells were untreated (Nil), treated with thrombin (0.2 U/mL; T) for 15 minutes, pretreated with inhibitor for 15 minutes, or pretreated with inhibitor followed by thrombin. FITC-dextran passage (μg/mL) during 30 minutes is shown. (A) Chelerythrine chloride (CC; 1 μM) blocks thrombin-stimulated permeability increases in ECs. (B) Calphostin C (CalC; 100 nM) does not block thrombin-stimulated permeability increases in ECs. (C) Bisindolylmaleimide I blocks thrombin-stimulated permeability increases in ECs at high concentrations (BisHi; 6 μM) but not at low concentrations (BisLo; 100 nM). Error bars indicate SEM.